Investigation of the Effect of Cisplatin on Fetuin A in the SH-SY5Y Cell Line
Yazarlar: Wissam ALKALİSH1, Yasir UBEYİD1, Abdurrahman HASAN1, Ahmet Kemal FİLİZ2, Ziad JOHA3
Corresponding author
1Wissam ALKALİSH, Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Dönem 4, Sivas, Türkiye
E-mail: wissamalkalesh@
Other authors
2Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Fizyoloji Anabilim Dalı, Sivas, Türkiye
3Sivas Cumhuriyet Üniversitesi Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Sivas, Türkiye
ÖZET
Amaç: Fetuin-A, esas olarak karaciğer ve yağ dokusunda sentezlenen ve daha sonra kan dolaşımına salınan bir glikoproteindir. Fetuin-A, farklı mekanizmalarla tümör ilerlemesi, kalsifikasyon, diyabet, obezite, böbrek hastalıkları ve koroner arter hastalığında önemli roller oynar. Fetuin-A, tümör hücrelerine endositoz yoluyla alınır ve hücre içindeyken eksozomların salgılanmasını artırır. Son zamanlarda yapılan bir dizi çalışma, prostat, pankreas ve multiform glioblastoma gibi tümör hücrelerinin bir alt kümesinin, ilerlemelerini destekleyen ektopik fetuin-A’yı sentezlediğini göstermiştir. Sisplatin, anti- kanser bir kemoterapi ilacıdır. Bu ilaç bir alkilleyici ajan olarak sınıflandırılır. Birkaç kanseri tedavi etmek için kullanılır. Bunlar meme kanseri, testis kanseri, mesane kanseri, mezotelyoma, serviks kanseri, over kanseri, akciğer kanseri, baş ve boyun kanseri, özefagus kanseri, beyin tümörleri ve nöroblastomayı içerir. Bu çalışmanın amacı sisplatinin SH-SY5Y hücre hattındaki fetuin A düzeyine etkisini araştırmaktır.
Yöntem: Bu çalışmada insan nöroblastoma (SH-SY5Y) hücre hatı kullanıldı. Sisplatinin fetuin A düzeyine etkisini araştırmak için iki hücre grubu hazırlandı. Kontrol grubu herhangi bir tedavi görmedi. Sisplatin grubundaki hücreler 24 saat 10 μM sisplatin ile muamele edildi. Hücreler donduruldu ve eksplozyona uğrayana kadar 3 kez çözüldü. Daha sonra santrifüj edildi ve süpernatanlar toplandı. Daha sonra fetuin A düzeyini belirlemek için ELISA (enzyme-linked immuno sorbent assay) yöntemi uygulandı.
Bulgular: Sisplatin grubunda kontrol grubuna göre fetuin A düzeyinde anlamlı bir artış saptandı. (p < 0,05)
Sonuçlar: Sisplatinin SH-SY5Y hücre hattında fetuin A seviyesini arttırdığı bulundu.
Anahtar Kelimeler: Fetuin A, Sisplatin.
ABSTRACT
Objective: Fetuin-A is a glycoprotein synthesized mainly in the liver and adipose tissue and then released into the bloodstream. Fetuin-A plays important roles in tumor progression, calcification, diabetes, obesity, kidney diseases and coronary artery disease through different mechanisms. Fetuin-A is taken into tumor cells by endocytosis, and while inside the cells, it increases the secretion of exosomes. Lately a number of studies have shown that a subset of tumor cells such as prostate, pancreatic and glioblastoma multiform synthesize ectopic fetuin-A, which promotes their progression. Cisplatin is an anti-cancer chemotherapy drug. This medication is classified as an alkylating agent. It is used to treat a number of cancers. These include breast cancer, testicular cancer, bladder cancer, mesothelioma, cervical cancer, ovarian cancer, lung cancer, head and neck cancer, esophageal cancer, brain tumors and neuroblastoma. The aim of this study was to investigate the effect of cisplatin on the fetuin A level in the SH-SY5Y cell line.
Method: In this study, the SH-SY5Y human neuroblastoma cell line was used. Two cell groups were prepared to investigate the effect of cisplatin on the fetuin A level. The control group did not undergo any treatment. Cells in the cisplatin group were treated with 10 μM cisplatin for 24 hours. Cells were frozen and re-thawed 3 times to burst. It was then centrifuged and the supernatants were collected. After that ELISA (enzyme-linked immunosorbent assay) method was applied to determine the level of fetuin A. Statistical evaluation of the data was performed by independent t-test, Statistical significance was defined at p<0.05.
Results: There was a significant increase in fetuin A level in the cisplatin group compared to the control. (p <0.05).
Conclusions: Cisplatin was found to increase the level of fetuin A in the SH-SY5Y cell line.
Keywords: Fetuin A, Cisplatin.
How to Cite (APA 7)
Alkalish, W., Ubeyid Y., Hasan A., Filiz A. K., Joha Z. (2022). Sisplatin’in SH-SY5Y Hücre Hattında Fetuin A’nın Üzerine Etkisinin Araştırılması. Health Sciences Student Journal, 2(3), 54-58. https://www.healthssj.com/sisplatinnin-sh-sy5y-hucre-hattinda-fetuin-anin-uzerine-etkisinin-arastirilmasi/
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