Investigation of the neuroprotective effect of hydralazine on PTZ-induced neurotoxicity in the SH-SY5Y cell line
Yazarlar: Said Yasin ŞİMŞEK1, Ahmet Turan İNCE2, Fatih YULAK3,4
Corresponding author
1Said Yasin Şimşek, Sivas Cumhuriyet Üniversitesi Tıp Fakültesi Dönem 2, Sivas, Türkiye
E-mail: saidyasinsimsek@gmail.com
Other authors
2Sivas Cumhuriyet Üniversitesi Tıp Fakültesi Dönem 3, Sivas, Türkiye
3Sivas Cumhuriyet Üniversitesi Tıp Fakültesi Fizyoloji Anabilim Dalı, Sivas, Türkiye
4Sivas Cumhuriyet Üniversitesi Tıp Fakültesi Araştırma Merkezi (CÜTFAM)
ÖZET
Amaç: Akut nöbet, bir grup kortikal nöronun aşırı ve senkronize deşarjlarına bağlı olarak ortaya çıkan ve klinikte gelip geçici olmakla birlikte hipokampusta nöron kaybına neden olan önemli bir nörolojik rahatsızlıktır. Oksidatif stres, nöbet sonrası meydana gelen hipokampal hasarlanmada etkin role sahiptir. Bu çalışmanın amacı, SH-SY5Y hücre hattında pentilentetrazol (PTZ) ile oluşturulan akut nöbet modeline hidralazin uygulayarak sinir koruyucu etkilerini araştırmaktır.
Yöntem: SH-SY5Y hücre kültüründe kontrol grubu, PTZ grubu, hidralazin grubu ve hidralazin+PTZ grubu olmak üzere 4 grup planlanmıştır. Kontrol grubuna herhangi bir uygulama yapılmazken hidralazin+PTZ grubuna çeşitli konsantrasyonlarda (6.25; 12.5; 25; 50 ve 100 µg/ml) hidralazin uygulanıp 24 saat sonra PTZ uygulanmıştır. XTT hücre canlılığı testi ile ilacımızın toksisite üzerine etkisi belirlenmiştir. Hücre süpernatantlarındaki doku TOS konsantrasyonları otomatik test yöntemi ile ölçülmüştür. Analizin verimliliği için hidrojen peroksit kullanılmıştır.
Bulgular: XTT hücre canlılığı sonuçları değerlendirildiğinde, hidralazin, toksisite oluşturulan SH-SY5Y hücrelerinde sinirsel (nöronal) canlılığı etkilememiştir. Ek olarak, hidralazin TOS seviyeleri üzerinde de kayda değer bir etki yapmamıştır (P>0.05).
Sonuçlar: Elde edilen veriler, çalıştığımız dozlardaki hidralazinin, PTZ ile indüklenen SH-SY5Y hücre hattı nörotoksisitesinde sinir koruyucu etkiye sahip olmadığını göstermiştir.
Anahtar Kelimeler: Hidralazin, nörotoksisite, PTZ, SH-SY5Y hücre hattı.
ABSTRACT
Objective: Acute seizure is an important neurological disorder that occurs due to excessive and synchronized discharges of a group of cortical neurons and causes neuronal loss in the hippocampus, although it is clinically temporary. Oxidative stress has an effective role in hippocampal damage that occurs after seizures. The aim of this study is to investigate the neuroprotective effects of hydralazine in an acute seizure model created with pentylenetetrazole (PTZ) in the SH-SY5Y cell line.
Method: In the SH-SY5Y cell culture, 4 groups were planned as control group, PTZ group, hydralazine group and hydralazine + PTZ group. While no application was made to the control group, hydralazine was applied to the hydralazine + PTZ group at various concentrations (6.25; 12.5; 25; 50 and 100 µg / ml) and PTZ was applied 24 hours later. The effect of our drug on toxicity was determined by XTT cell viability test. Tissue TOS concentrations in cell supernatants were measured by the automated test method. Hydrogen peroxide was used for the efficiency of the analysis.
Results: When evaluating XTT cell viability results, hydralazine did not affect neuronal viability in toxicity induced SH-SY5Y cells. In addition, hydralazine did not have a significant effect on TOS levels (P>0.05).
Conclusions: The data obtained showed that hydralazine at the doses we studied did not have a neuroprotective effect on the SH-SY5Y cell line neurotoxicity induced by PTZ.
Keywords: Hydralazine, neurotoxicity, PTZ, SH-SY5Y cell line.
How to Cite (APA 7)
Simsek, S. Y., Ince, A. T., Yulak, F. (2021). Hidralazinin SH-SY5Y hücre hattında PTZ ile indüklenen nörotoksisite üzerindeki nöroprotektif etkisinin araştırılması. Health Sciences Student Journal, 1(1), 1-6. https://healthssj.com/hidralazinin-sh-sy5y-hucre-hattinda-ptz-ile-induklenen-norotoksisite-uzerindeki-noroprotektif-etkisinin-arastirilmasi/
KAYNAKÇA/REFERENCES
1. Çalışma, E., Moderatörleri, G., Naz, S., Prof, Y., & Gürses, C. (n.d.). Epilepsi Çalışma Grubu Tanı ve Tedavi Rehberi 2015 TÜRK NÖROLOJİ DERNEĞİ 2015.
2. Taskiran, A. S., Ergul, M., Gunes, H., Ozturk, A., Sahin, B., & Ozdemir, E. (2020). The Effects of Proton Pump Inhibitors (Pantoprazole) on Pentylenetetrazole-Induced Epileptic Seizures in Rats and Neurotoxicity in the SH-SY5Y Human Neuroblastoma Cell Line. Cellular and Molecular Neurobiology. https://doi.org/10.1007/s10571-020-00956-6
3. Lamberty, Y., & Klitgaard, H. (2000). Consequences of Pentylenetetrazole Kindling on Spatial Memory and Emotional Responding in the Rat. Epilepsy and Behavior, 1(4), 256–261. https://doi.org/10.1006/ebeh.2000.0085
4. Bolterman, R. J., Manriquez, M. C., Ortiz Ruiz, M. C., Juncos, L. A., & Romero, J. C. (2005). Effects of captopril on the renin angiotensin system, oxidative stress, and endothelin in normal and hypertensive rats. Hypertension, 46(4), 943–947. https://doi.org/10.1161/01.HYP.0000174602.59935.d5
5. Ramos, S. F., Mendonça, B. P., Leffa, D. D., Pacheco, R., Damiani, A. P., Hainzenreder, G., Petronilho, F., Dal-Pizzol, F., Guerrini, R., Calo’, G., Gavioli, E. C., Boeck, C. R., & De Andrade, V. M. (2012). Effects of neuropeptide S on seizures and oxidative damage induced by pentylenetetrazole in mice. Pharmacology Biochemistry and Behavior, 103(2), 197–203. https://doi.org/10.1016/j.pbb.2012.09.001
6. Potschka, H. (2012). Animal models of drug-resistant epilepsy. Epileptic Disorders, 14(3), 226–234. https://doi.org/10.1684/epd.2012.0532
7. kalantaripour, T. pari, Esmaeili-Mahani, S., Sheibani, V., Najafipour, H., & Asadi-Shekaari M, M. (2017). Apelin-13 protects rat primary cortical glia-neuron co-culture against pentylenetetrazole-induced toxicity. Biomedicine and Pharmacotherapy, 87, 661–668. https://doi.org/10.1016/j.biopha.2016.12.131
8. McComb, M. N., Chao, J. Y., & Ng, T. M. H. (2016). Direct Vasodilators and Sympatholytic Agents. In Journal of Cardiovascular Pharmacology and Therapeutics (Vol. 21, Issue 1, pp. 3–19). SAGE Publications Ltd. https://doi.org/10.1177/1074248415587969
9. Taşkıran, A , Ergül, M . (2021). The Protective Effect of Hydralazine against Hydrogen Peroxide (H2O2)-Induced Oxidative Damage in C6 Glial Cell Line . Turkish Journal of Science and Health, 2(1) , 8-15 . Retrieved from https://dergipark.org.tr/tr/pub/tfsd/issue/60096/787868
10. Erel, O. (2004). A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical Biochemistry, 37(2), 112–119. https://doi.org/10.1016/j.clinbiochem.2003.10.014
11. Guo, X., Han, C., Ma, K., Xia, Y., Wan, F., Yin, S., Kou, L., Sun, Y., Wu, J., Hu, J., Huang, J., Xiong, N., & Wang, T. (2019). Hydralazine Protects Nigrostriatal Dopaminergic Neurons From MPP+ and MPTP Induced Neurotoxicity: Roles of Nrf2-ARE Signaling Pathway. Frontiers in Neurology, 10(March), 1–13. https://doi.org/10.3389/fneur.2019.00271
12. Fujii, K., Weno, B. L., Baumbach, G. L., & Heistad, D. D. (1992). Effect of antihypertensive treatment on focal cerebral infarction. Hypertension, 19(6), 713–716. https://doi.org/10.1161/01.HYP.19.6.713
13. Shi, R., Page, J. C., & Tully, M. (2015). Molecular mechanisms of acrolein-mediated myelin destruction in CNS trauma and disease. Free Radical Research, 49(7), 888–895. https://doi.org/10.3109/10715762.2015.1021696
14. Mehrabani, M., Nematollahi, M. H., Esmaili, M., Bahrampour, K., Abolhassani, M., Sharifi, A. M., Pasban, H., Saravani, M., & Mirzamohammadi, S. (2020). Protective effect of Hydralazine on a cellular model of Parkinson’s disease: a possible role of hypoxia-inducible factor (HIF)-1α. Biochemistry and Cell Biology, 1–34. https://doi.org/10.1139/bcb-2019-0117
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