REM ve Total Uyku Yoksunluklarının İnflamatuar Bağırsak Hastalığına Etkisinin Araştırılması

Abstract

Aim: Sleep deprivation has recently become an important public health problem. Numerous studies have proposed a complex relationship between sleep, inflammation and the immune system. Inadequate sleep is common in patients with inflammatory bowel disease (IBD) and is associated with disease activity. Although previous studies have shown that total sleep deprivation negatively affects the disease process, no studies have been conducted on the effect of REM sleep deprivation alone on IBD. In this study, we aimed to evaluate the effect of REM sleep elimination on the IBD process and to compare the effect of total sleep deprivation.

Method: In this study, 24 Balb/c strain mice were randomly divided into 4 groups: 1. control group, 2. DSS group; group exposed to 2.0% Dextran Sodium Sulphate (DSS) dissolved in drinking water for 7 days, 3. DSS + REMSD group; group exposed to 2.0% DSS dissolved in drinking water for 7 days and REM sleep deprivation, 4. DSS + TSD group; group exposed to 2.0% DSS dissolved in drinking water for 7 days and total sleep deprivation. After animal weights and disease activity index were determined for one week, plasma CRP levels, colon lengths and damage to the colon were measured.

Results: Glutamate decreased cell survival and TAS levels, but increased TOS and caspase-3 levels. Although myostatin treatment alone partially increased antioxidant levels, it had no significant effect when co-treated with glutamate.

Conclusion: These results suggest that sleep deprivation may exacerbate colitis activation by triggering inflammatory processes.